Synthesis of cellulose by Acetobacter Xylinum. 4. Enzyme systems present in a crude extract of glucose-grown cells.
نویسندگان
چکیده
Brown, S. A. & Neish, A. C. (1954). Canad. J. Biochem. Physiol. 32, 170. Butlin, K. R. (1938). Biochem. J. 32, 508.. Edelman, J., Ginzburg, V. & Hassid, W. Z. (1955). J. biol. Chem. 213, 843. Fitting, C. & Doudoroff, M. (1952). J. biol.'Chem. 199, 153. Friedemann, T. E. & Haugen, G. E. (1943). J. biol. Chem. 147, 415. Greathouse, G. A. (1953). Science, 117, 553. Greathouse, G. A., Shirk, H. G. & Minor, F. W. (1954). J. Amer. chem. Soc. 76,5157. Gromet, Z., Schramm, M. & Hestrin, S. (1957). Biochem. J. 67, 679. Hestrin, S., Aschner, M. & Mager, J. (1947). Nature, Lond., 159, 64. Hestrin, S. & Schramm, M. (1954). Biochem. J. 58, 345. Horrocks, R. H. (1949). Nature, Lond., 164, 444. Jeanes, A., Wise, C. S. &]Dimler, R. J. (1951). Analyt. Chem. 23, 415. Jones, A. R., Dowling, E. J. & Skraba, W. J. (1953). Analyt. Chem. 25, 394. Katznelson, H., Tanenbaum, S. W. & Tatum, E. L. (1953). J. biol. Chem. 204,43. Kaushal, R. & Walker, T. K. (1951). Biochem. J. 48, 618. Khouvine, Y. (1936). Bull. Soc. Chim. biol., Paris, 18, 1325. Khym, J. & Cohn, W. (1953). J. Amer. chem. Soc. 75,1153. Kluyver, A. & Boezaardt, A. G. J. (1938). Rec. Trav. chim. Pays-Bas, 57, 609. Kulka, D. & Walker, T. K. (1954). Arch. Biochem. Biophys. 50, 169. Lanning, M. C. & Cohen, S. S. (1951). J. biol. Chem. 189,109. Macleod, M. & Robison, R. (1929). Biochem. J. 28, 517. Mager, J. & Aschner, M. (1947). J. Bact. 53, 283. Muhlethaler, K. (1949). Biochim. biophy8. Acta, 3, 527. Nelson, N. (1944). J. biol. Chem. 153, 357. Preston, R. D. & Astbury, W. T. (1954). Nature, Lond., 173, 203. Preston, R. D. & Kuyper, D. (1951). J. exp. Bot. 2, 247. Ranby, B. G. (1952). Ark. Kemi, 14, 249. Schramm, M. (1956). Analyt. Chem. 28, 963. Sohramm, M., Gromet, Z. & Hestrin, S. (1957). Nature, Lond., 179, 28. Schramm, M. & Hestrin, S. (1954a). J. gen. Microbiol. 11, 123. Schramm, M. & Hestrin, S. (1954b). Biochem. J. 56, 163. Shafizadeh, F. & Wolfrom, M. L. (1955). J. Amer. chem. Soc. 77, 5182. Stacey, M. (1954). Advane. Enzymol. 15, 315. Stephenson, M. (1949). Bacterial Metaboi87m, p. 107. London: Longmans, Green and Co. Steward, F. C. & Muhlethaler, K. (1953). Ann. Bot., N.S., 17, 295. Steward, F. C. & Muhlethaler, K. (1954). Nature, Lond., 178, 203. Tarr, H. L. A. & Hibbert, H. (1931). Canad. J. Res. 4, 372. Ujejski, L. & Waygood, E. R. (1954). Canad. J. Chem. 32, 14. Wood, W. A. (1955). Bact. Rev. 19, 222. Wright, H. B. & Walker, T. K. (1955). Chem. & Ind. p. 18.
منابع مشابه
Synthetic Medium for Acetobacter xylinum That Can Be Used for Isolation of Auxotrophic Mutants and Study of Cellulose Biosynthesis.
Acetobacter xylinum is a bacterium that can synthesize cellulose when grown in an undefined medium containing glucose. We developed a defined minimal medium for A. xylinum that contains 1% glucose, 0.1% NH(4)Cl, 0.115% citric acid, 0.33% Na(2)HPO(4), 0.01% KCl, 0.025% MgSO(4). 7H(2)O, and 7.5 mg of nicotinamide per liter which both allows cellulose synthesis and can be used to isolate a variety...
متن کاملThe synthesis of cellulose in cell-free extracts of Acetobacter xylinum.
In a previous publication (2) evidence was presented that the synthesis of chitin in Neurospora crassa occurs by way of a glycosyl transfer from uridine diphosphate-N-acetylglucosamine. The similarity of the structure of chitin and cellulose suggested that a like mechanism may be operative in the biosynthesis of cellulose. It has been shown by Hestrin and Schramm (3) that non-viable lyophilized...
متن کاملRegulation of hexose phosphate metabolism in Acetobacter xylinum.
The metabolism of glucose and fructose was studied in resting succinate-grown cells of Acetobacter xylinum. From fructose only cellulose and CO(2) were formed by the cells, whereas from glucose, gluconate was formed much more rapidly than these two products. The molar ratio of sugar converted into cellulose to sugar converted into CO(2) was significantly greater than unity for both hexoses. The...
متن کاملPyruvate-phosphate dikinase and the control of gluconeogenesis in Acetobacter xylinum.
Ryruvate-phosphate dikmase formation was induced on transfer of glucose-grown cells of Acefobacfer xylinum to succinateor pyruvate-containing media. Enzyme formation did not occur if such media were supplemented with glucose or in the presence of chloramphenicol. Fructose and glycerol were less effective in repressing enzyme formation. When glucose was added to cultures of cells growing on succ...
متن کاملIntermediatry steps in Acetobacter xylinum cellulose synthesis: studies with whole cells and cell-free preparations of the wild type and a celluloseless mutant.
Intermediatry steps in cellulose synthesis in Acetobacter xylinum were studied with resting cells and particulate-membranous preparations of the wild-type strain and of a celluloseless mutant. Exogenously supplied [1-14C]glucose was rapidly converted by resting cells of both types into glucose 6-phosphate, glucose 1-phosphate, and uridine glucose 5'-diphosphate (UDP)-glucose and incorporated in...
متن کاملCellulose biosynthesis in Acetobacter xylinum: visualization of the site of synthesis and direct measurement of the in vivo process.
In vivo synthesis of cellulose by Acetobacter xylinum was monitored by darkfield light microscopy. Cellulose is synthesized in the form of a ribbon projecting from the pole of the bacterial rod. The ribbon elongates at a rate of 2 mum min-1. The ribbon consists of approximately 46 microfibrils which average 1.6 X 5.8 nm in cross section. The observed microfibrillar elongation rate corresponds t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 67 4 شماره
صفحات -
تاریخ انتشار 1957